Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSF2 Double Nickase Plasmid (h): sc-402400-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSF2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSF2 Double Nickase Plasmid (h) and HSF2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSF2 Antibody (G-11): sc-74529
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSF2 Double Nickase Plasmid (h) | sc-402400-NIC | 20 µg | $410.00 | |||
HSF2 Double Nickase Plasmid (h2) | sc-402400-NIC-2 | 20 µg | $410.00 |
HSF2 (heat shock factor 2) is a sequence-specific transcription factor that coordinates stress-responsive gene expression and contributes to proteostasis by regulating heat shock proteins and molecular chaperone networks. Beyond classical heat shock signaling, HSF2 participates in developmental and differentiation programs, including neuronal and germ cell pathways, and interfaces with chromatin remodeling to shape stimulus-dependent transcriptional outputs. HSF2 activity can modulate cell-cycle progression, apoptosis susceptibility, and responses to proteotoxic stress, linking it to pathways relevant to neurodegeneration, cancer biology, and disorders characterized by impaired protein homeostasis. Dysregulated HSF2-dependent transcriptional programs have been reported across multiple disease-relevant contexts, supporting its use as a mechanistic node for studying stress adaptation and transcriptional regulation in human cells.
HSF2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.