HSF1 Double Nickase Plasmid (h): sc-400432-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSF1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSF1 Double Nickase Plasmid (h) and HSF1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSF1 Antibody (E-4): sc-17757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSF1 Double Nickase Plasmid (h) | sc-400432-NIC | 20 µg | $410.00 | |||
HSF1 Double Nickase Plasmid (h2) | sc-400432-NIC-2 | 20 µg | $410.00 |
Human HSF1 (heat shock factor 1) is a master transcriptional regulator of the heat shock response that coordinates inducible expression of molecular chaperones such as HSP70 and HSP90 to maintain proteostasis during cellular stress. Upon proteotoxic stress, HSF1 undergoes trimerization and nuclear accumulation, binds heat shock elements (HSEs), and drives programs affecting protein folding, quality control, and stress-adaptive metabolism. HSF1 activity interfaces with pathways regulating apoptosis, autophagy, and the unfolded protein response, shaping cellular resilience to oxidative, thermal, and metabolic insults. Dysregulated HSF1 signaling has been implicated in proteostasis disorders and in stress-adaptation phenotypes observed across diverse disease models, supporting mechanistic studies of stress-responsive transcriptional networks.
HSF1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.