HNF-4 alpha Anticuerpo (H-1) recomendado para detectarHNF-4α de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

HNF-4α Anticuerpo (H-1): sc-374229

HNF-4α Anticuerpo (H-1) clasificado 3.9 de 5 por 14.
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Ficha técnica

    • Anti-HNF-4 alpha Anticuerpo H-1 es un monoclonal de ratón IgG1 κ HNF-4α Anticuerpo, ver las 32 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 295-465 de HNF-4α de origen human
    • HNF-4 alpha Anticuerpo (H-1) es recomendado para detectar HNF-4α de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • HNF-4 alpha Anticuerpo (H-1) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • TransCruz reactivo para Gel Supershift y ChIP (sc-374229 X, 200 µg/0.1 ml)
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HNF-4α (H-1): sc-374229.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HNF-4α Antibody (H-1) for WB and IHC(P) applications. These reagents are now offered in bundles with HNF-4α Antibody (H-1) (see ordering information below).
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Twelve HNF4a isoforms are described in litterature (Hui Ling Ko et al, Cell Reports, 2019). Which isoform(s) recongnize sc-374229 antibody?

Preguntado por: Maude
Thank you for your question. HNF-4α Antibody (H-1): sc-374229 was raised against amino acids 295-465 of HNF-4α of human origin. We do not have any data indicating which isoforms the antibody recognizes based on this immunogenic sequence.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-12-28

Can we request a trial size of the HNF4 (H-1) (sc-374229)? We have been testing several antibody combinations to try to visualize HCC in mouse tissue while co-staining. So far, many of the goat and rat antibodies have not worked at all. Thank you.

Preguntado por: Ashley Haddock
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Respondida por: Technical Support
Fecha de publicación: 2020-05-06

What is the method (steps) of applying this clone as immunohistochemical marker to the paraffin embedded sections, please?

Preguntado por: Dodo123
Thank you for your question. Here is a link to our recommended protocol for IHC on paraffin tissue sections: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Respondida por: Tech Service
Fecha de publicación: 2020-02-17

How much antibody do I get in an order of sc-374229? All I see on the webpage is the concentration of the antibody (200 ug/ml). Do you send a ml? 

Preguntado por: cfb6
Thank you for your question. Yes, this antibody, sc-374229, is provided as 200µg of antibody in 1ml.
Respondida por: Tech Service
Fecha de publicación: 2019-06-24

Can the TransCruz reagent sc-374229 X be used in applications other than ChIP and EMSA?

Preguntado por: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used in other applications such as Western Blot, IF and IHC. The dilution factor needs to be adjusted as the X form is ten times as concentrated as the standard antibody.
Respondida por: Technical Service
Fecha de publicación: 2017-01-27
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Rated 1 de 5 por de Not working in mouse liver by IHC/IF I didn't get any stains in the nucleus in mouse liver at all with 1:50 by both IHC and IF. I tried Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse 2nd abs, but still no signals. I contacted with the technical support. They shared me some references, no replacements and no solutions.
Fecha de publicación: 2021-06-30
Rated 2 de 5 por de Not working in MIN6 cells We performed an IHC in MIN6 cells (mouse) with the concentrated antibody of HNF4a (diluted 10x times to match the normal concentration) and we obtained no signal in 1:50 and 1:100 concentration. We know these cells are expressing HNF4a and the antibody should be working with mouse cells according to the datasheet. We already tested a free sample of HNF1a and the results were better, with some signal in this case. Can you help is with troubleshooting? HNF4a antibody could be dead? Kind regards, Pablo.
Fecha de publicación: 2021-04-27
Rated 4 de 5 por de Complutense University School of Medicine We use this antibody for IF staining with HepaRG undifferentiated cells experiment which sounds good, but not HepG2 cells.
Fecha de publicación: 2019-06-07
Rated 1 de 5 por de No signal at all in mouse liver Although HNF-4a is a well-established hepatocyte marker this antibody did not stain mouse liver sections. We tested it in paraffin-embedded mouse liver sections using PT Link (DAKO) High pH, PT Link Low pH and citrate buffer for antigen retrieval. We tested it in mouse liver cryosections too. Dilutions tested were 1:100 and 1:500. None of this conditions showed any nuclear staining in hepatocytes. Can you help me with troubleshooting? Thank you, TR22
Fecha de publicación: 2018-03-12
Rated 5 de 5 por de good work well for IHC, it marks the hepatocytes in liver section.
Fecha de publicación: 2017-11-14
Rated 4 de 5 por de A good Antibody I got a free sample a month ago and are happy that it was work on western blot in mice liver tissues. We had ordered this Ab.
Fecha de publicación: 2017-10-06
Rated 4 de 5 por de 效果不错 同样的,HNF-4a,本人也是申请的小样抗体,目前只跑了WB,1:500的浓度稀释,条带还不错,感觉有点淡,会有扎带,但不是很明显
Fecha de publicación: 2017-09-22
Rated 3 de 5 por de 感觉不怎么样 之前订购的是羊的多抗,但是现在下架了,然后用的是鼠的单抗,在肝癌和肠癌里面做了个对比,1:1000的稀释比,和以前的实验结果相比,条带是干净,但是没有以前的好显,而且现在一些肠癌里面都显不出来了,这可能和单抗没有多抗的识别位点多有关吧,建议能够解决这个问题。
Fecha de publicación: 2017-06-13
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