HMX2 CRISPR/Cas9 KO Plasmid (m): sc-420886
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HMX2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HMX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMX2 CRISPR/Cas9 KO Plasmid (m) | sc-420886 | 20 µg | $397.00 |
Overview
Hmx2 encodes the homeobox transcription factor HMX2, a nuclear DNA-binding protein that regulates gene expression programs during embryonic development. In mouse, HMX2 is implicated in patterning and differentiation of sensory structures, with prominent roles in inner ear morphogenesis and vestibular system formation through transcriptional control of lineage specification. By coordinating developmental transcriptional networks, HMX2 influences cell fate decisions, tissue architecture, and maturation of specialized epithelia. Disruption of Hmx2-related pathways is relevant to studying congenital defects affecting auditory and balance function and broader mechanisms of developmental dysregulation.
HMX2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hmx2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hmx2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hmx2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HMX2 protein expression.
This CRISPR knockout system enables efficient generation of Hmx2-deficient cell models for investigation of HMX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hmx2 exon(s) critical for HMX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hmx2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HMX2 CRISPR/Cas9 KO Plasmid (m) and HMX2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hmx2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HMX2 HDR Plasmid (m) and HMX2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hmx2 homology arms to support homology-directed repair at defined Hmx2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.