Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HIV Tat-SF1 CRISPR/Cas9 KO Plasmid (h): sc-404479
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HIV Tat-SF1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HIV Tat-SF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HIV-1 Tat-SF1 Antibody (C-4): sc-514351
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV Tat-SF1 CRISPR/Cas9 KO Plasmid (h) | sc-404479 | 20 µg | $397.00 |
Overview
HTATSF1 encodes HIV Tat-specific factor 1 (HIV Tat-SF1), a nuclear RNA-binding protein that functions as a transcriptional cofactor and regulator of pre-mRNA processing. HIV Tat-SF1 has been linked to coordination of RNA polymerase II transcription with spliceosome activity, influencing alternative splicing and mRNA maturation across diverse gene programs. Through these roles in co-transcriptional RNA processing, HTATSF1 contributes to cellular homeostasis and has been studied in the context of host–virus interactions that depend on precise control of transcription and RNA metabolism. Dysregulation of transcription-coupled splicing factors is broadly relevant to disease-associated changes in gene expression networks and RNA processing phenotypes.
HIV Tat-SF1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HTATSF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HTATSF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HTATSF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HIV Tat-SF1 protein expression.
This CRISPR knockout system enables efficient generation of HTATSF1-deficient cell models for investigation of HIV Tat-SF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HTATSF1 exon(s) critical for HIV Tat-SF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HTATSF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HIV Tat-SF1 CRISPR/Cas9 KO Plasmid (h) and HIV Tat-SF1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HTATSF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HIV Tat-SF1 HDR Plasmid (h) and HIV Tat-SF1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HTATSF1 homology arms to support homology-directed repair at defined HTATSF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.