Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h): sc-403221-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h) and Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HDAC10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone Deacetylase 10 (HDAC10) Antibody (E-2): sc-393417
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h) | sc-403221-NIC | 20 µg | $410.00 | |||
Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h2) | sc-403221-NIC-2 | 20 µg | $410.00 |
HDAC10 encodes Histone Deacetylase 10, a zinc-dependent histone deacetylase that modulates chromatin accessibility and transcriptional programs by removing acetyl groups from histones and other protein substrates. As a class II deacetylase with prominent cytoplasmic roles, HDAC10 has been linked to regulation of autophagy, DNA damage responses, and cellular stress adaptation, influencing pathways that control proliferation and survival. Altered HDAC10 expression or activity has been associated with changes in epigenetic state and metabolic and immune-related signaling networks observed across multiple disease contexts, including cancer biology and neuroinflammatory processes. These functions make HDAC10 a useful target for dissecting epigenetic control of gene expression and its downstream phenotypes in human cell models.
Histone Deacetylase 10 (HDAC10) Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HDAC10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HDAC10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HDAC10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HDAC10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.