HIF1a Antibody (28b): sc-13515
- HIF1a Antibody (28b) is a mouse monoclonal IgG1 κ HIF1a antibody, cited in 290 publications, provided at 200 µg/ml
- epitope mapping within amino acids 329-530 of HIF-1α of human origin
- HIF1a Antibody (28b) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including and bovine and porcine
- Anti-HIF1a Antibody (28b) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-13515 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HIF1a Antibody (28b) for WB and IHC(P) applications. These reagents are now offered in bundles with HIF1a Antibody (28b) (see ordering information below).
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HIF-1α Antibody (28b) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the hypoxia-inducible factor 1-alpha (HIF-1α) protein, with epitope mapping within amino acids 329-530 of human HIF-1α. HIF-1α monoclonal antibody (28b) is indispensable for researchers investigating cellular mechanisms of oxygen deprivation, as HIF-1α, also known as HIF1, MOP1, PASD8, and HIF-1alpha, is a pivotal transcription factor that regulates expression of genes involved in glycolysis, erythropoiesis, and angiogenesis, thereby playing a crucial role in restoring oxygen homeostasis and maintaining cell growth and viability under hypoxic conditions. HIF-1α monoclonal antibody (28b) exhibits broad species reactivity, effectively detecting HIF-1α in mouse, rat, and human samples, as well as in additional species including bovine and porcine, across various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Available in both non-conjugated form and multiple conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and several Alexa Fluor® conjugates, HIF-1α monoclonal antibody (28b) provides versatile options to accommodate diverse experimental requirements. By enabling precise detection and study of HIF-1α, HIF-1α monoclonal antibody (28b) supports research into regulation of hypoxia-responsive genes and intricate pathways that cells utilize to adapt to low oxygen environments.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIF1a Antibody (28b) References:
- PKB/Akt activation inhibits p53-mediated HIF1A degradation that is independent of MDM2. | Choy, MK., et al. 2010. J Cell Physiol. 222: 635-9. PMID: 19950214
- STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy. | Ferreira, JV., et al. 2013. Autophagy. 9: 1349-66. PMID: 23880665
- Distinct roles of HIF1A in endothelial adaptations to physiological and ambient oxygen. | Jiang, YZ., et al. 2014. Mol Cell Endocrinol. 391: 60-7. PMID: 24796659
- Golgi Associated HIF1a Serves as a Reserve in Melanoma Cells. | Lakhter, AJ., et al. 2016. J Cell Biochem. 117: 853-9. PMID: 26375488
- LIMS1 Promotes Pancreatic Cancer Cell Survival under Oxygen-Glucose Deprivation Conditions by Enhancing HIF1A Protein Translation. | Huang, C., et al. 2019. Clin Cancer Res. 25: 4091-4103. PMID: 30679163
- Protein co-expression network-based profiles revealed from laser-microdissected cancerous cells of lung squamous-cell carcinomas. | Nishimura, T., et al. 2021. Sci Rep. 11: 20209. PMID: 34642392
- CARM1 drives triple-negative breast cancer progression by coordinating with HIF1A. | Feng, D., et al. 2024. Protein Cell. 15: 744-765. PMID: 38476024
- HIF1A protein expression is correlated with clinical features in gastric cancer: an updated systematic review and meta-analysis. | Nam, S. and Lee, Y. 2024. Sci Rep. 14: 13736. PMID: 38877062
- Quercetin relieves compression-induced cell death and lumbar disc degeneration by stabilizing HIF1A protein. | Ren, J., et al. 2024. Heliyon. 10: e37349. PMID: 39296087
- RNA binding protein ELAVL1-mediated USP33 stabilizes HIF1A to promote pathological proliferation, migration and angiogenesis of RECs. | Xie, J., et al. 2024. Int Ophthalmol. 44: 393. PMID: 39320536
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIF1a Antibody (28b) | sc-13515 | 200 µg/ml | $322.00 | |||
HIF1a Antibody (28b): m-IgG Fc BP-HRP Bundle | sc-528283 | 200 µg Ab; 10 µg BP | $361.00 | |||
HIF1a Antibody (28b): m-IgGκ BP-HRP Bundle | sc-520622 | 200 µg Ab, 40 µg BP | $361.00 | |||
HIF1a Antibody (28b) X | sc-13515 X | 200 µg/0.1 ml | $322.00 | |||
HIF1a Antibody (28b) AC | sc-13515 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HIF1a Antibody (28b) HRP | sc-13515 HRP | 200 µg/ml | $322.00 | |||
HIF1a Antibody (28b) FITC | sc-13515 FITC | 200 µg/ml | $336.00 | |||
HIF1a Antibody (28b) PE | sc-13515 PE | 200 µg/ml | $349.00 | |||
HIF1a Antibody (28b) Alexa Fluor® 488 | sc-13515 AF488 | 200 µg/ml | $364.00 | |||
HIF1a Antibody (28b) Alexa Fluor® 546 | sc-13515 AF546 | 200 µg/ml | $364.00 | |||
HIF1a Antibody (28b) Alexa Fluor® 594 | sc-13515 AF594 | 200 µg/ml | $364.00 | |||
HIF1a Antibody (28b) Alexa Fluor® 647 | sc-13515 AF647 | 200 µg/ml | $364.00 | |||
HIF1a Antibody (28b) Alexa Fluor® 680 | sc-13515 AF680 | 200 µg/ml | $364.00 | |||
HIF1a Antibody (28b) Alexa Fluor® 790 | sc-13515 AF790 | 200 µg/ml | $364.00 |