HIF-1 alpha Anticuerpo (28b) recomendado para detectarHIF-1α de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)

HIF-1α Anticuerpo (28b): sc-13515

HIF-1α Anticuerpo (28b) clasificado 4.2 de 5 por 77.
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Ficha técnica

    • Anti-HIF-1 alpha Anticuerpo 28b es un monoclonal de ratón IgG1 κ HIF-1α Anticuerpo, ver las 180 publicaciones, proporcionado como 200 µg/ml
    • epitope mapping within amino acids 329-530 of HIF-1α of human origin
    • HIF-1 alpha Anticuerpo (28b) es recomendado para detectar HIF-1α de mouse, rat y human origen, mediante WB, IP, IF y IHC(P); también reactivo con otras especies, incluyendo anyd bovine and porcine
    • HIF-1 alpha Anticuerpo (28b) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • TransCruz reactivo para Gel Supershift y ChIP (sc-13515 X, 200 µg/0.1 ml)
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HIF-1α (28b): sc-13515.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HIF-1α Antibody (28b) for WB and IHC(P) applications. This reagent is now offered in a bundle with HIF-1α Antibody (28b) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

what is the starting dilution for HIF-1 # (28b) and HIF-1 (H 1# 67) using IHC formalin fixed human tissues

Preguntado por: Anónimo
Thank you for your question. You can find this information in the antibodies' datasheets, which are available from their respective product pages. The recommended starting dilution for IHC applications is 1:50, with a dilution range between 1:50 and 1:500 depending on your samples and pre-treatment procedures.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-08-04

你好,可以做小鼠脑片的免疫荧光吗?效果如何?

Preguntado por: xiaomimi
Thank you for your question. sc-13515 is recommended for detection of HIF-1α by IF on mouse sample.Please contact our Technical Service Department for more details. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问,sc-13515 推荐免疫荧光的实验检测小鼠样本。 更多问题请请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Respondida por: Technical Support 9
Fecha de publicación: 2020-03-25

パラフィン切片を免疫組織化学染色する際は、どのような抗原賦活化を行えばよいですか?

Preguntado por: seigo
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to answer any questions. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Respondida por: Technical Support 15
Fecha de publicación: 2019-06-05

请问这个做肿瘤切片染色,需要二抗吗?

Preguntado por: Curry
感谢您的提问。免疫组化实验中,如果使用HIF-1α (28b): sc-13515,需要使用相应二抗检测。或者可购买HRP偶联的HIF-1α (28b): sc-13515,货号为sc-13515 HRP,则不需要二抗。 Thank you for your inquiry. If you perform IHC with HIF-1α (28b): sc-13515, a matched secondary antibody is necessary. Or you can choose HRP conjugated primary antibody:sc-13515 HRP.
Respondida por: Technical Support 9
Fecha de publicación: 2019-05-22

Does it work in bEnd3 endothelial cells. Actually i applied one another HIF1a antibodies(goat) , it worked in 1:200 . So if anyone deal with bEnd3 cells, please contact me . Thank you

Preguntado por: endothelial
Thank you for your question. HIF-1α Antibody (28b): sc-13515 is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including and bovine and porcine. You can find several publications using this antibody with murine cells on its product page.
Respondida por: SCBT Heidelberg Support
Fecha de publicación: 2018-12-29

Hi there  Please could you tell me if this antibody HIF-1α Antibody (28b): sc-13515 has worked in frozen skin sections for IF and if so what is the protocol and fixation step you use  Thanks

Preguntado por: Kous
Thank you for your question. We are not aware of any product citations specifically using this antibody in frozen skin sections for IF. Our recommended protocol is provided below as a reference. Please contact our Technical Service Department for help with searching the many product citations for this antibody (by application or sample/cell type). https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Technical Support
Fecha de publicación: 2018-12-19

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue in paraffin sections?

Preguntado por: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-13515 is available directly conjugated to FITC, AF488, and AF647.
Respondida por: TechService7
Fecha de publicación: 2016-12-16

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Preguntado por: Cweed
In such cases, we recommend using a directly conjugated mouse monoclonal primary antibody. This product, sc-13515, is available directly conjugated to FITC, AF488, and AF647.
Respondida por: TechService7
Fecha de publicación: 2016-12-16
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Rated 4 de 5 por de Antibody Received good results using this antibody. Used in murine tissue (paraffin-embedded).
Fecha de publicación: 2020-09-29
Rated 5 de 5 por de Perfect staining in IHC Paraffin embedded human jejunal sections (n=6; 5 µm) were rehydrated, antigen retrieval was performed heating sections 25 minutes at 90 °C in a water bath in the presence of 10 mmol / l sodium citrate buffer pH 6.0. Sections were treated with an endogenous peroxidase blocking kit. Immunostaining was performed using a commercial kit following the manufacturer's instructions. Ab Dilution: 0.02 µg/ µl In the jejunum of control samples (full thickness), the antibody works well and give a specific staining of vascular components (endothelium). This marker stains also many enterocytes. Some muscle cell belonging to the longitudinal muscle (external) layer were mild positive.
Fecha de publicación: 2020-07-01
Rated 2 de 5 por de Not good in WB exp in CHO Very weak signal at correct size and a lot of unspecific bands with equal strength in WB Exp. in CHO cells
Fecha de publicación: 2020-01-15
Rated 5 de 5 por de the function is good i am happy to buy the antibody and it is good for WB
Fecha de publicación: 2019-08-14
Rated 1 de 5 por de Using breast cancer cells it didn't work in WB exp I tried this antibody for western blotting applications, on MCF7 cells cultured for 8, 24, 48 and 72 hours in normoxia or hypoxia (1% O2) but I didn't get the right band. I used 1:100-1:200 and 1:1000 dilution in 1% milk dissolved in PBS-0.1%Tween incubated over-night at 4°C. I barely detected a band, and when so I could only visualize a lower molecular weight band, possibly aspecific, as it was not affected by the cultivation in hypoxia. Even with positive control (SK-MEL-28 cells cultured in 1% O2 fro 24 hours) I never got a band. -> In our hands, not a good antibody.
Fecha de publicación: 2018-06-05
Rated 2 de 5 por de WB Hif-1a (28b) did not worked for mouse lung primary fibroblast WB. We used anti-mouse IgG1.
Fecha de publicación: 2018-03-28
Rated 1 de 5 por de No nuclear staining in mouse paraffin sections Antibody stains hipoxic areas (close to necrosis) without nuclear staining.
Fecha de publicación: 2018-03-15
Rated 5 de 5 por de good IHC - P 1:100 it is good and background is clear
Fecha de publicación: 2018-01-31
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