Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hic-5 CRISPR/Cas9 KO Plasmid (h): sc-402786
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hic-5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hic-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hic-5 Antibody (C-6): sc-271353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hic-5 CRISPR/Cas9 KO Plasmid (h) | sc-402786 | 20 µg | $397.00 |
Overview
TGFB1I1 encodes Hic-5, a focal adhesion and nuclear LIM domain adaptor that coordinates integrin-dependent signaling with transcriptional responses to mechanical stress. Hic-5 scaffolds protein complexes involved in actin cytoskeleton remodeling, adhesion turnover, and Rho-family GTPase signaling, influencing cell spreading, migration, and extracellular matrix interactions. It also interfaces with TGF-β–responsive programs and related pathways that shape fibrotic and epithelial–mesenchymal transition–like phenotypes. Dysregulated Hic-5 activity has been associated with altered stromal remodeling and invasive cell behavior in multiple disease-relevant contexts, supporting its use as a mechanotransduction and transcriptional co-regulation target in human cell models.
Hic-5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TGFB1I1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TGFB1I1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TGFB1I1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hic-5 protein expression.
This CRISPR knockout system enables efficient generation of TGFB1I1-deficient cell models for investigation of Hic-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TGFB1I1 exon(s) critical for Hic-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TGFB1I1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hic-5 CRISPR/Cas9 KO Plasmid (h) and Hic-5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TGFB1I1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hic-5 HDR Plasmid (h) and Hic-5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TGFB1I1 homology arms to support homology-directed repair at defined TGFB1I1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.