HES1 Double Nickase Plasmid (h): sc-400387-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HES1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HES1 Double Nickase Plasmid (h) and HES1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HES1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HES1 Antibody (E-5): sc-166410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HES1 Double Nickase Plasmid (h) | sc-400387-NIC | 20 µg | $410.00 | |||
HES1 Double Nickase Plasmid (h2) | sc-400387-NIC-2 | 20 µg | $410.00 |
HES1 encodes a basic helix-loop-helix transcriptional repressor that functions as a canonical downstream effector of Notch signaling, where it dampens lineage-specific transcriptional programs to maintain progenitor states. By binding N-box/E-box motifs and recruiting corepressor complexes, HES1 helps coordinate cell-cycle control, differentiation timing, and tissue patterning in neural, endocrine, and hematopoietic contexts. HES1 activity integrates with pathways such as Wnt, Hedgehog, and TGF-β to tune fate decisions and oscillatory transcriptional dynamics during development. Dysregulated HES1 expression or Notch–HES1 axis activity has been linked to aberrant differentiation and proliferation programs observed across multiple disease-relevant models, including developmental disorders and oncogenic signaling contexts.
HES1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HES1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HES1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HES1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HES1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.