HDAC8 Anticuerpo (E-5) recomendado para detectar HDAC8 de human origen, mediante WB, IP, IF, IHC(P) y ELISA

HDAC8 Anticuerpo (E-5): sc-17778

HDAC8 Anticuerpo (E-5) clasificado 4.3 de 5 por 6.
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Ficha técnica

    • Anti-HDAC8 Anticuerpo E-5 es un monoclonal de ratón IgG2b (kappa light chain) HDAC8 Anticuerpo proporcionado como 200 µg/ml
    • producido contra los amino ácidos 1-145 de HDAC8 de origen human
    • HDAC8 Anticuerpo (E-5) es recomendado para detectar HDAC8 de human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • HDAC8 Anticuerpo (E-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HDAC8 (E-5): sc-17778.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HDAC8 Antibody (E-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with HDAC8 Antibody (E-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can HDAC8 (E-5): sc-17778 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HDAC8 (E-5): sc-17778 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 2 de 5 por de not work well for my experiment we repeated the experiment more than three times and we did not detect the band on the predict position (1:500, rat heart extrat)
Fecha de publicación: 2017-09-21
Rated 5 de 5 por de High Quality The HDAC8 Antibody (E-5): sc-17778 works very well for immunofluorescence applications, showing strong staining with nuclear localization. This antibody has also been cited multiple times for further verification.
Fecha de publicación: 2017-02-08
Rated 5 de 5 por de Good antibody for Immunofluorescence staining Good antibody for Immunofluorescence staining of methanol-fixed MOLT-4 cells showing nuclear localization. - SCBT QC
Fecha de publicación: 2015-04-27
Rated 5 de 5 por de Clean blot with single band in human HDAC8 Clean blot with single band in human HDAC8 transfected 293T whole cell lysates by WB. - SCBT QC
Fecha de publicación: 2015-04-15
Rated 4 de 5 por de Weak bands in non Weak bands in non-transfected control and HDAC8 siRNA transfected HeLa cells. - SCBT QC
Fecha de publicación: 2015-03-05
Rated 5 de 5 por de Single strong band with a little background Single strong band with a little background for western blot analysis of HDAC8 expression in K-562 nuclear extract. - SCBT QC
Fecha de publicación: 2013-03-15
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