HDAC11 Anticuerpo (C-5) recomendado para detectarHDAC11 de mouse, rat y human origen, mediante WB, IP, IF y ELISA

HDAC11 Anticuerpo (C-5): sc-390737

HDAC11 Anticuerpo (C-5) clasificado 4.4 de 5 por 5.
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Ficha técnica

    • Anti-HDAC11 Anticuerpo C-5 es un monoclonal de ratón IgG2b (kappa light chain) HDAC11 Anticuerpo proporcionado como 200 µg/ml
    • producido contra los amino ácidos 1-300 localizados en el N-terminus de HDAC11 de origen human
    • HDAC11 Anticuerpo (C-5) es recomendado para detectar HDAC11 de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • HDAC11 Anticuerpo (C-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HDAC11 (C-5): sc-390737.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HDAC11 Antibody (C-5) for WB applications. This reagent is now offered in a bundle with HDAC11 Antibody (C-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Using this antibody in WB(8% agarose gel), which one is the right band, about 37kDa or 50kDa?

Preguntado por: jm lee
Thank you for your question. The predicted molecular weight for HDAC11 is approximately 39 kDa. However, we would expect the observed molecular weight to be slightly higher than the predicted molecular weight. Unfortunately, we do not have any references to support a molecular weight as high as 50 kDa. We will review the data using this antibody on our website and make updates very shortly to remove any unsupportive data.
Respondida por: Technical Support
Fecha de publicación: 2017-08-30

If using the Li-Cor system, what is the recommended secondary for this antibody?

Preguntado por: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Tech Service
Fecha de publicación: 2017-01-30
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Rated 4 de 5 por de Good antibody for WB. Test this antibody by WB using mouse lung cancer cell lysates and it works well although there are non-specific bands.
Fecha de publicación: 2017-09-12
Rated 4 de 5 por de a relative good antibody for WB This antibody generates a clear signal at the predicted position on the western blot (1:500 dilution, 40ug HeLa extract).
Fecha de publicación: 2017-09-01
Rated 5 de 5 por de Great HDAC11 monoclonal choice, strong WB bands! Highly specific strong band in Caki-1 whole cell lysate.
Fecha de publicación: 2017-01-10
Rated 5 de 5 por de Used this antibody in WB Used this antibody in WB, worked well, did not see any non specific bands, happy with purchase.
Fecha de publicación: 2015-08-17
Rated 4 de 5 por de Good specificity and signal strength by Western Good specificity and signal strength by Western in Caki-1 whole cell lysate. -SCBT QC
Fecha de publicación: 2014-11-01
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