HAPLN1 CRISPR/Cas9 KO Plasmid (m): sc-419816
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAPLN1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HAPLN1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAPLN1 CRISPR/Cas9 KO Plasmid (m) | sc-419816 | 20 µg | $397.00 |
Overview
Hapln1 encodes hyaluronan and proteoglycan link protein 1 (HAPLN1), an extracellular matrix (ECM) component that stabilizes hyaluronan–proteoglycan aggregates and supports the structural organization of pericellular matrices. By reinforcing interactions between hyaluronan and chondroitin sulfate proteoglycans, HAPLN1 contributes to cartilage and connective tissue integrity and influences cell adhesion, mechanotransduction, and matrix-dependent signaling. Its activity is closely tied to ECM assembly and remodeling processes that intersect with pathways governing tissue development, inflammation, and fibrosis. Dysregulated HAPLN1-associated matrix architecture has been implicated in degenerative joint phenotypes and broader stromal alterations that can modulate cell migration and tissue homeostasis.
HAPLN1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hapln1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hapln1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hapln1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HAPLN1 protein expression.
This CRISPR knockout system enables efficient generation of Hapln1-deficient cell models for investigation of HAPLN1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hapln1 exon(s) critical for HAPLN1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hapln1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HAPLN1 CRISPR/Cas9 KO Plasmid (m) and HAPLN1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hapln1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HAPLN1 HDR Plasmid (m) and HAPLN1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hapln1 homology arms to support homology-directed repair at defined Hapln1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.