hamartin Antibody (C-8): sc-377386
- hamartin Antibody (C-8) is a mouse monoclonal IgG2b κ hamartin antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of hamartin of human origin
- hamartin Antibody (C-8) is recommended for detection of hamartin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-hamartin Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hamartin Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with hamartin Antibody (C-8) (see ordering information below).
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hamartin Antibody (C-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects hamartin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). hamartin (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. hamartin (C-8) monoclonal antibody recognizes a protein that plays a crucial role in regulating cell growth and proliferation, particularly in the context of tuberous sclerosis complex (TSC), an autosomal dominant genetic disorder that leads to the formation of benign tumors known as hamartomas. hamartin, encoded by the tuberous sclerosis-1 gene (TSC1), forms a complex with tuberin, the product of the TSC2 gene, which is essential for inhibiting the mTOR pathway, a key regulator of cellular metabolism and growth. Disruption of this complex can lead to uncontrolled cell growth and tumor development, highlighting hamartin′s importance in maintaining cellular homeostasis. hamartin expression is widespread in adult tissues, with particularly high levels found in the brain, heart, and kidney, underscoring its significance in various physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hamartin Antibody (C-8) References:
- The expression of hamartin, the product of the TSC1 gene, in normal human tissues and in TSC1- and TSC2-linked angiomyolipomas. | Plank, TL., et al. 1999. Mod Pathol. 12: 539-45. PMID: 10349994
- Characterization of the cytosolic tuberin-hamartin complex. Tuberin is a cytosolic chaperone for hamartin. | Nellist, M., et al. 1999. J Biol Chem. 274: 35647-52. PMID: 10585443
- The TSC1 tumour suppressor hamartin regulates cell adhesion through ERM proteins and the GTPase Rho. | Lamb, RF., et al. 2000. Nat Cell Biol. 2: 281-7. PMID: 10806479
- A new twist for the tumour suppressor hamartin. | Fukuhara, S. and Gutkind, JS. 2000. Nat Cell Biol. 2: E76-8. PMID: 10806489
- Tuberin and hamartin expression is reduced in the majority of subependymal giant cell astrocytomas in tuberous sclerosis complex consistent with a two-hit model of pathogenesis. | Jóźwiak, S., et al. 2004. J Child Neurol. 19: 102-6. PMID: 15072102
- The tuberous sclerosis gene products hamartin and tuberin are multifunctional proteins with a wide spectrum of interacting partners. | Rosner, M., et al. 2008. Mutat Res. 658: 234-46. PMID: 18291711
- Endogenous neuroprotection: hamartin modulates an austere approach to staying alive in a recession. | Hadley, G., et al. 2013. Int J Stroke. 8: 449-50. PMID: 23879750
- Hamartin: An Endogenous Neuroprotective Molecule Induced by Hypoxic Preconditioning. | Li, S., et al. 2020. Front Genet. 11: 582368. PMID: 33193709
- Identification and characterization of the tuberous sclerosis gene on chromosome 16. | ,. 1993. Cell. 75: 1305-15. PMID: 8269512
- Identification of the tuberous sclerosis gene TSC1 on chromosome 9q34. | van Slegtenhorst, M., et al. 1997. Science. 277: 805-8. PMID: 9242607
- The genetic basis of tuberous sclerosis. | Young, J. and Povey, S. 1998. Mol Med Today. 4: 313-9. PMID: 9743993
- Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene, interacts with tuberin and appears to be localized to cytoplasmic vesicles. | Plank, TL., et al. 1998. Cancer Res. 58: 4766-70. PMID: 9809973
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hamartin Antibody (C-8) | sc-377386 | 200 µg/ml | $322.00 | |||
hamartin Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-523306 | 200 µg Ab, 40 µg BP | $361.00 | |||
hamartin Antibody (C-8): m-IgG2b BP-HRP Bundle | sc-549104 | 200 µg Ab; 10 µg BP | $361.00 | |||
hamartin Antibody (C-8) AC | sc-377386 AC | 500 µg/ml, 25% agarose | $424.00 | |||
hamartin Antibody (C-8) HRP | sc-377386 HRP | 200 µg/ml | $322.00 | |||
hamartin Antibody (C-8) FITC | sc-377386 FITC | 200 µg/ml | $336.00 | |||
hamartin Antibody (C-8) PE | sc-377386 PE | 200 µg/ml | $349.00 | |||
hamartin Antibody (C-8) Alexa Fluor® 488 | sc-377386 AF488 | 200 µg/ml | $364.00 | |||
hamartin Antibody (C-8) Alexa Fluor® 546 | sc-377386 AF546 | 200 µg/ml | $364.00 | |||
hamartin Antibody (C-8) Alexa Fluor® 594 | sc-377386 AF594 | 200 µg/ml | $364.00 | |||
hamartin Antibody (C-8) Alexa Fluor® 647 | sc-377386 AF647 | 200 µg/ml | $364.00 | |||
hamartin Antibody (C-8) Alexa Fluor® 680 | sc-377386 AF680 | 200 µg/ml | $364.00 | |||
hamartin Antibody (C-8) Alexa Fluor® 790 | sc-377386 AF790 | 200 µg/ml | $364.00 |