Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GRPR Double Nickase Plasmid (h): sc-401637-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRPR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GRPR Double Nickase Plasmid (h) and GRPR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRPR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRPR Antibody (D-1): sc-398549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRPR Double Nickase Plasmid (h) | sc-401637-NIC | 20 µg | $410.00 | |||
GRPR Double Nickase Plasmid (h2) | sc-401637-NIC-2 | 20 µg | $410.00 |
Human GRPR (gastrin-releasing peptide receptor) is a G protein–coupled receptor that binds gastrin-releasing peptide/neuromedin B family ligands to activate PLCβ-dependent signaling, intracellular calcium mobilization, and downstream MAPK/ERK and PI3K pathway responses. GRPR activity influences epithelial and neuronal cell communication, regulating processes such as secretion, smooth muscle contractility, and mitogenic signaling. Altered GRPR expression or signaling has been reported in multiple tumor contexts and is also relevant to neuroendocrine biology, making it a useful node for studying GPCR-driven transcriptional programs, receptor desensitization/internalization, and calcium-dependent signaling networks.
GRPR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRPR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRPR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRPR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRPR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.