GRASP65 Antibody (D-12): sc-374423
- GRASP65 Antibody (D-12) is a mouse monoclonal IgG1 κ GRASP65 antibody, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 347-385 near the C-terminus of GRASP65 of human origin
- GRASP65 Antibody (D-12) is recommended for detection of GRASP65 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GRASP65 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GRASP65 Antibody (D-12) for WB applications. These reagents are now offered in bundles with GRASP65 Antibody (D-12) (see ordering information below).
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GRASP65 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects GRASP65 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-GRASP65 antibody (D-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GRASP65 plays a crucial role in the structure and function of the Golgi apparatus, a vital organelle responsible for processing and packaging proteins and lipids for secretion or delivery to other organelles. GRASP65 is part of the Golgi stacking machinery, working alongside GRASP55 to maintain the integrity of the Golgi structure through the formation of cisternal stacks. This stacking is essential for proper functioning of the Golgi, as stacking facilitates efficient transport of proteins through the secretory pathway. The interaction between GRASP65 and other golgins, as well as involvement in dynamic processes of Golgi reassembly during cell division, underscores GRASP65′s importance in cellular organization and protein trafficking. Using GRASP65 (D-12) antibody in research can provide valuable insights into mechanisms of Golgi function and implications in various cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GRASP65 Antibody (D-12) References:
- GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system. | Shorter, J., et al. 1999. EMBO J. 18: 4949-60. PMID: 10487747
- Transmembrane transforming growth factor-alpha tethers to the PDZ domain-containing, Golgi membrane-associated protein p59/GRASP55. | Kuo, A., et al. 2000. EMBO J. 19: 6427-39. PMID: 11101516
- Mitotic phosphorylation of Golgi reassembly stacking protein 55 by mitogen-activated protein kinase ERK2. | Jesch, SA., et al. 2001. Mol Biol Cell. 12: 1811-7. PMID: 11408587
- A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic. | Short, B., et al. 2001. J Cell Biol. 155: 877-83. PMID: 11739401
- Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus. | Barr, FA., et al. 2001. J Cell Biol. 155: 885-91. PMID: 11739402
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRASP65 Antibody (D-12) | sc-374423 | 200 µg/ml | $322.00 | |||
GRASP65 Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-527462 | 200 µg Ab; 10 µg BP | $361.00 | |||
GRASP65 Antibody (D-12): m-IgG1 BP-HRP Bundle | sc-532835 | 200 µg Ab; 20 µg BP | $361.00 | |||
GRASP65 Antibody (D-12) AC | sc-374423 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GRASP65 Antibody (D-12) HRP | sc-374423 HRP | 200 µg/ml | $322.00 | |||
GRASP65 Antibody (D-12) FITC | sc-374423 FITC | 200 µg/ml | $336.00 | |||
GRASP65 Antibody (D-12) PE | sc-374423 PE | 200 µg/ml | $349.00 | |||
GRASP65 Antibody (D-12) Alexa Fluor® 488 | sc-374423 AF488 | 200 µg/ml | $364.00 | |||
GRASP65 Antibody (D-12) Alexa Fluor® 546 | sc-374423 AF546 | 200 µg/ml | $364.00 | |||
GRASP65 Antibody (D-12) Alexa Fluor® 594 | sc-374423 AF594 | 200 µg/ml | $364.00 | |||
GRASP65 Antibody (D-12) Alexa Fluor® 647 | sc-374423 AF647 | 200 µg/ml | $364.00 | |||
GRASP65 Antibody (D-12) Alexa Fluor® 680 | sc-374423 AF680 | 200 µg/ml | $364.00 | |||
GRASP65 Antibody (D-12) Alexa Fluor® 790 | sc-374423 AF790 | 200 µg/ml | $364.00 | |||
GRASP65 (D-12) Neutralizing Peptide | sc-374423 P | 100 µg/0.5 ml | $69.00 |