GRAP1 Antibody (E-7): sc-398199

GRAP1 Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects GRAP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRAP1, also known as GRASP-1 or GRIP-associated protein, plays a crucial role in the regulation of Ras signaling pathways and the distribution of AMPA receptors, which are vital for synaptic transmission and plasticity in the nervous system. GRAP1 contains a rasGEF domain, a caspase-3 cleavage site, a ras binding domain, and a PDZ domain, which together facilitate function as a GDP/GTP exchange factor. The caspase-3 cleavage site is particularly significant as it separates the GEF catalytic domain from the regulatory domain, allowing for precise control of GRAP1′s activity. This structural organization is essential for maintaining proper localization and function of GRAP1, as GRAP1 is primarily expressed in the cytosol and partially localized to the membrane across all nervous system tissues. Furthermore, GRAP1 interacts with the PDZ domain of GRIP-1, an adapter protein that links AMPA receptors to other signaling molecules, thereby influencing receptor trafficking and synaptic function. The ability of caspase-3 to cleave GRAP1 may disrupt regulatory mechanisms, highlighting the importance of post-translational modifications in GRAP1′s functionality and signaling pathways.

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GRAP1 Antibody (E-7) References:

1. Interaction of GRASP, a protein encoded by a novel retinoic acid-induced gene, with members of the cytohesin family of guanine nucleotide exchange factors.  |  Nevrivy, DJ., et al. 2000. J Biol Chem. 275: 16827-36. PMID: 10828067
2. GRASP-1: a neuronal RasGEF associated with the AMPA receptor/GRIP complex.  |  Ye, B., et al. 2000. Neuron. 26: 603-17. PMID: 10896157
3. Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes.  |  Stinton, LM., et al. 2005. Clin Immunol. 116: 108-17. PMID: 15897011
4. GRASP-1 is a neuronal scaffold protein for the JNK signaling pathway.  |  Ye, B., et al. 2007. FEBS Lett. 581: 4403-10. PMID: 17761173
5. Dual roles for lysine 490 of promyelocytic leukemia protein in the transactivation of glucocorticoid receptor-interacting protein 1.  |  Liu, ST., et al. 2013. Biochim Biophys Acta. 1833: 1799-810. PMID: 23542129
6. Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.  |  Goodarzi, H., et al. 2016. Cell. 165: 1416-1427. PMID: 27259150
7. GRASP1 Regulates Synaptic Plasticity and Learning through Endosomal Recycling of AMPA Receptors.  |  Chiu, SL., et al. 2017. Neuron. 93: 1405-1419.e8. PMID: 28285821
8. A novel RBMX-TFE3 gene fusion in a highly aggressive pediatric renal perivascular epithelioid cell tumor.  |  Argani, P., et al. 2020. Genes Chromosomes Cancer. 59: 58-63. PMID: 31408245
9. Analysis of deubiquitinase OTUD5 as a biomarker and therapeutic target for cervical cancer by bioinformatic analysis.  |  Bai, M., et al. 2020. PeerJ. 8: e9146. PMID: 32655987
10. Analysis of Expression of the GRIPAP1, DLG4, KIF1B, NGFRAP1, and NRF1 Genes in Peripheral Blood of the Patients with Parkinson's Disease in the Early Clinical Stages.  |  Lukashevich, MV., et al. 2024. Biochemistry (Mosc). 89: 1779-1788. PMID: 39523115

The preferred GRAP1 antibody is GRAP1 Antibody (A-6): sc-398198.