Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPRC5B CRISPR/Cas9 KO Plasmid (h): sc-409271
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPRC5B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPRC5B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPRC5B Antibody (A-8): sc-518246
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPRC5B CRISPR/Cas9 KO Plasmid (h) | sc-409271 | 20 µg | $397.00 |
Overview
GPRC5B (G protein-coupled receptor class C group 5 member B) is an orphan GPCR-like membrane protein implicated in signal integration at the cell surface, with reported roles in regulating MAPK/ERK and PI3K-AKT pathway outputs, receptor trafficking, and transcriptional responses to extracellular cues. It is expressed in multiple tissues and has been linked to control of cell proliferation, differentiation, and metabolic signaling, including insulin-responsive processes in adipose and muscle contexts. Altered GPRC5B expression has been associated with inflammatory signaling and oncogenic phenotypes in several model systems, suggesting relevance to studies of tumor biology, metabolic disease mechanisms, and immune-related pathways. As a membrane-associated regulator, it is frequently investigated for its impact on receptor cross-talk, cell signaling dynamics, and stress-adaptive programs.
GPRC5B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPRC5B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPRC5B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPRC5B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPRC5B protein expression.
This CRISPR knockout system enables efficient generation of GPRC5B-deficient cell models for investigation of GPRC5B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPRC5B exon(s) critical for GPRC5B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPRC5B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPRC5B CRISPR/Cas9 KO Plasmid (h) and GPRC5B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPRC5B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPRC5B HDR Plasmid (h) and GPRC5B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPRC5B homology arms to support homology-directed repair at defined GPRC5B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.