GPR34 CRISPR Activation Plasmid (m): sc-423884-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR34 CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- GPR34 CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by GPR34 CRISPR Activation Plasmid (m) and GPR34 CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Gpr34 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR34 CRISPR Activation Plasmid (m) | sc-423884-ACT | 20 µg | $397.00 | |||
GPR34 CRISPR Activation Plasmid (m2) | sc-423884-ACT-2 | 20 µg | $397.00 |
Mouse Gpr34 encodes GPR34, a class A rhodopsin-like G protein-coupled receptor enriched in immune and myeloid lineages, including microglia. GPR34 signaling has been linked to modulation of chemotaxis, inflammatory tone, and cAMP-dependent GPCR pathways that shape innate immune activation and tissue-resident macrophage behavior. In the central nervous system, GPR34 contributes to microglial homeostasis and responses to injury-associated cues, intersecting with neuroinflammatory processes. Altered GPCR signaling through GPR34 has been investigated in contexts of inflammatory disorders and tumor-associated myeloid biology, supporting its use as a probe for immune regulation mechanisms.
GPR34 CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Gpr34 expression without altering the underlying DNA sequence.
GPR34 CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Gpr34 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Gpr34 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous GPR34 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Gpr34 locus and enabling the study of GPR34-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of GPR34 pathway restoration in tumor cells with silenced or reduced Gpr34 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.