GPR22 CRISPR/Cas9 KO Plasmid (m): sc-428483
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR22 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR22 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR22 CRISPR/Cas9 KO Plasmid (m) | sc-428483 | 20 µg | $397.00 |
Overview
Gpr22 encodes GPR22, an orphan G protein-coupled receptor predominantly studied in cardiac and vascular biology, where it is linked to regulation of stress-responsive signaling and cellular remodeling programs. Although its endogenous ligand remains unclear, GPCR-like coupling suggests potential influence on second messenger pathways such as cAMP/PKA and MAPK signaling that shape transcriptional responses, metabolism, and contractile function. In mouse models and expression studies, GPR22 has been associated with cardiovascular phenotypes including cardiac hypertrophy and heart failure–related remodeling, motivating investigation of its role in cardiomyocyte and vascular cell homeostasis. Gpr22 is also used as a marker in tissue-specific transcriptional profiling to connect GPCR signaling states with disease-relevant gene networks.
GPR22 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gpr22 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gpr22 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gpr22 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR22 protein expression.
This CRISPR knockout system enables efficient generation of Gpr22-deficient cell models for investigation of GPR22 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gpr22 exon(s) critical for GPR22 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gpr22 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR22 CRISPR/Cas9 KO Plasmid (m) and GPR22 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gpr22 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR22 HDR Plasmid (m) and GPR22 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gpr22 homology arms to support homology-directed repair at defined Gpr22 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.