Glycophorin A Antibody (YTH89.1): sc-59182

Glycophorin A Antibody (YTH89.1) is a rat monoclonal IgG2b antibody that detects Glycophorin A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunohistochemistry, and flow cytometry (FCM). Glycophorin A is a crucial sialoglycoprotein located in the human erythrocyte membrane, where Glycophorin A plays a significant role in maintaining the structural integrity of red blood cells and facilitating cell-cell interactions. Glycophorin A spans the membrane once, with its amino-terminal end exposed to the extracellular environment, which is essential for Glycophorin A′s function in defining blood group phenotypes through antigenic determinants. The Glycophorin A gene, located on chromosome 4q31.21, consists of seven exons and encodes a 150 amino acid protein that is 97% homologous to Glycophorin B, highlighting the evolutionary relationship between these proteins. The presence of Glycophorin A on the erythrocyte surface is vital for the recognition of blood group antigens, which can influence transfusion compatibility and susceptibility to certain diseases. Anti-Glycophorin A antibody (YTH89.1) is an invaluable tool for researchers studying erythrocyte biology, blood group genetics, and related clinical applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Glycophorin A Antibody (YTH89.1) References:

1. Thermodynamics of glycophorin A transmembrane helix dimerization in C14 betaine micelles.  |  Fleming, KG., et al. 2004. Biophys Chem. 108: 43-9. PMID: 15043920
2. Glycophorin A dimerization is driven by specific interactions between transmembrane alpha-helices.  |  Lemmon, MA., et al. 1992. J Biol Chem. 267: 7683-9. PMID: 1560003
3. Design of a functional membrane protein by engineering a heme-binding site in glycophorin A.  |  Cordova, JM., et al. 2007. J Am Chem Soc. 129: 512-8. PMID: 17227013
4. Expression of human glycophorin A in wild type and glycosylation-deficient Chinese hamster ovary cells. Role of N- and O-linked glycosylation in cell surface expression.  |  Remaley, AT., et al. 1991. J Biol Chem. 266: 24176-83. PMID: 1748686
5. Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function.  |  Khoory, J., et al. 2016. PLoS One. 11: e0141206. PMID: 26784696
6. Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study.  |  Baldari, B., et al. 2021. Healthcare (Basel). 9: PMID: 33946627
7. A monoclonal antibody directed against the homologous N-terminal domain of glycophorin A and B.  |  Edelman, L., et al. 1984. Exp Clin Immunogenet. 1: 129-39. PMID: 6401002
8. The transmembrane domain of glycophorin A as studied by cross-linking using photoactivatable phospholipids.  |  Ross, AH., et al. 1982. J Biol Chem. 257: 4152-61. PMID: 7068629
9. Insertion of glycophorin A, a transmembraneous protein, in lipid bilayers can be mediated by electropermeabilization.  |  Raffy, S. and Teissie, J. 1995. Eur J Biochem. 230: 722-32. PMID: 7607245
10. Cooperative action between band 3 and glycophorin A in human erythrocytes: immobilization of band 3 induced by antibodies to glycophorin A.  |  Knowles, DW., et al. 1994. Biophys J. 66: 1726-32. PMID: 8061221

The preferred Glycophorin A antibody is Glycophorin A Antibody (R10): sc-53905.