Glucose Transporter Glut8 Double Nickase Plasmid (h): sc-403183-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glucose Transporter Glut8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glucose Transporter Glut8 Double Nickase Plasmid (h) and Glucose Transporter Glut8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC2A8. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucose Transporter Glut8 Double Nickase Plasmid (h) | sc-403183-NIC | 20 µg | $410.00 | |||
Glucose Transporter Glut8 Double Nickase Plasmid (h2) | sc-403183-NIC-2 | 20 µg | $410.00 |
SLC2A8 encodes glucose transporter Glut8 (GLUT8), a facilitative sugar transporter that contributes to cellular carbohydrate uptake and intracellular glucose distribution in a tissue- and context-dependent manner. GLUT8 activity supports metabolic homeostasis by influencing glycolytic flux, glycogen handling, and broader insulin-responsive nutrient transport programs, with downstream effects on energy sensing pathways such as AMPK and mTOR. Regulation of SLC2A8 expression and transporter trafficking has been studied in relation to endocrine and metabolic phenotypes, including insulin resistance and altered glucose handling. Because glucose transport constrains anabolic and redox demands, SLC2A8 is also relevant to mechanistic work on proliferation, differentiation, and stress adaptation in metabolically active cells.
Glucose Transporter Glut8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC2A8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC2A8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC2A8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC2A8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.