GGA1 Antibody (D-8): sc-390420

GGA1 Antibody (D-8) is a mouse monoclonal IgG3 antibody that detects GGA1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GGA1 is a crucial member of the GGA family of proteins, which are essential for the proper trafficking of soluble proteins from the trans-Golgi network to endosomes and lysosomes. This trafficking is vital for maintaining cellular homeostasis and ensuring that proteins reach their correct destinations within the cell. GGA1, along with GGA2 and GGA3, features a modular structure that includes an N-terminal VHS domain, a GAT domain, a hinge segment, and a C-terminal GAE domain, allowing GGA1 to interact with mannose 6-phosphate receptors and clathrin. The ability of GGA1 to recognize and bind to acidic-cluster di-leucine sequences in sorting receptors is critical for the sorting process, and GGA1′s colocalization with GGA2 on perinuclear membranes highlights GGA1′s role in cellular organization. The human GGA1 gene, located on chromosome 22, encodes a protein that shares significant sequence identity with GGA2 and GGA3, underscoring the evolutionary conservation and functional importance of GGA1 in intracellular transport mechanisms. Anti-GGA1 antibody (D-8) is an invaluable tool for researchers studying protein trafficking and cellular dynamics.

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GGA1 Antibody (D-8) References:

1. Autoinhibition of the ligand-binding site of GGA1/3 VHS domains by an internal acidic cluster-dileucine motif.  |  Doray, B., et al. 2002. Proc Natl Acad Sci U S A. 99: 8072-7. PMID: 12060753
2. Multiple phosphorylation events regulate the subcellular localization of GGA1.  |  McKay, MM. and Kahn, RA. 2004. Traffic. 5: 102-16. PMID: 14690499
3. GGA1 interacts with the adaptor protein AP-1 through a WNSF sequence in its hinge region.  |  Bai, H., et al. 2004. J Biol Chem. 279: 17411-7. PMID: 14973137
4. Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5.  |  Zhu, G., et al. 2004. EMBO J. 23: 3909-17. PMID: 15457209
5. Demonstration of BACE (beta-secretase) phosphorylation and its interaction with GGA1 in cells by fluorescence-lifetime imaging microscopy.  |  von Arnim, CA., et al. 2004. J Cell Sci. 117: 5437-45. PMID: 15466887
6. Clathrin adaptor GGA1 polymerizes clathrin into tubules.  |  Zhang, F., et al. 2007. J Biol Chem. 282: 13282-9. PMID: 17344219
7. Molecular basis for autoregulatory interaction between GAE domain and hinge region of GGA1.  |  Inoue, M., et al. 2007. Traffic. 8: 904-13. PMID: 17506864
8. GGA autoinhibition revisited.  |  Cramer, JF., et al. 2010. Traffic. 11: 259-73. PMID: 20015111
9. Correction: Clathrin adaptor GGA1 modulates myogenesis of C2C12 myoblasts.  |  Isobe, M., et al. 2018. PLoS One. 13: e0209441. PMID: 30543706
10. Total flavonoids of Rhizoma drynariae improves tendon-bone healing for anterior cruciate ligament reconstruction in mice and promotes the osteogenic differentiation of bone mesenchymal stem cells by the ERR1/2-Gga1-TGF-β/MAPK pathway.  |  Han, L., et al. 2024. Environ Toxicol. 39: 106-119. PMID: 37665165

The preferred GGA1 antibody is GGA1 Antibody (D-6): sc-271927.