GFAP Antibody (2A5): sc-65343

GFAP Antibody (2A5) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect glial fibrillary acidic protein (GFAP), an essential intermediate filament protein belonging to the type III subclass of intermediate filaments. GFAP, also known as ALXDRD, plays a crucial role in maintaining the structural integrity and functionality of astroglial cells within the central nervous system. GFAP is characterized by its tripartite structure, comprising an amino-terminal head domain, a central α-helical rod domain responsible for filament assembly, and a carboxy-terminal tail domain that facilitates interactions with other cellular proteins and organelles. Post-translational modifications of GFAP, particularly phosphorylation, are vital for regulating filament dynamics, assembly, and the cellular response to injury, making the detection of these modifications important for understanding astrocyte behavior in various pathological conditions. GFAP monoclonal antibody (2A5) is raised against purified porcine spinal cord GFAP and exhibits robust reactivity across multiple species, including mouse, rat, human, and porcine, making GFAP (2A5) antibody an ideal tool for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-GFAP antibody (2A5) is particularly valuable for studying astrogliosis, a process marked by increased GFAP expression in response to neurological insults such as mechanical trauma, AIDS related dementia, prion infections, and inflammatory demyelinating diseases, thereby providing critical insights into the mechanisms underlying central nervous system disorders. Available in a non-conjugated form, GFAP (2A5) monoclonal antibody is an indispensable reagent for researchers aiming to elucidate the complex roles of GFAP in neural health and disease, facilitating advanced studies into the molecular and cellular pathways that govern astrocyte function and response to injury.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GFAP Antibody (2A5) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-GFAP Antibody (2A5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GFAP Antibody (2A5) Specifications

Alternate Product Name: ALXDRD

Product Clone Name: 2A5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: spinal cord GFAP

Epitope Species: porcine

Product Reactivity/Detection: GFAP

Species Reactivity/Detection: mouse, rat, human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for GFAP Antibody (2A5): N/A

The preferred GFAP antibody is GFAP Antibody (2E1): sc-33673.