Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GAS41 CRISPR/Cas9 KO Plasmid (m): sc-425664
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GAS41 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GAS41 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GAS41 Antibody (C-10): sc-393708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAS41 CRISPR/Cas9 KO Plasmid (m) | sc-425664 | 20 µg | $397.00 |
Overview
Mouse Yeats4 encodes GAS41, a YEATS domain–containing chromatin-associated factor that participates in transcriptional regulation by linking histone acetylation signals to chromatin remodeling. GAS41 is reported to associate with epigenetic regulatory complexes, supporting RNA polymerase II–dependent gene expression programs that influence cell-cycle progression, DNA replication, and genome stability. Through its roles in chromatin organization and transcriptional control, Yeats4/GAS41 is relevant to studies of oncogenic transcriptional states, aneuploidy, and stress-response pathways in proliferative tissues. Dysregulation of YEATS family chromatin readers has been connected to altered enhancer activity and transformation-associated gene expression, making Yeats4 a useful node for mechanistic cancer and developmental biology research.
GAS41 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Yeats4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Yeats4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Yeats4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GAS41 protein expression.
This CRISPR knockout system enables efficient generation of Yeats4-deficient cell models for investigation of GAS41 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Yeats4 exon(s) critical for GAS41 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Yeats4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GAS41 CRISPR/Cas9 KO Plasmid (m) and GAS41 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Yeats4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GAS41 HDR Plasmid (m) and GAS41 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Yeats4 homology arms to support homology-directed repair at defined Yeats4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.