Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GAP-43 CRISPR/Cas9 KO Plasmid (m): sc-420484
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GAP-43 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GAP-43 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GAP-43 Antibody (B-5): sc-17790
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAP-43 CRISPR/Cas9 KO Plasmid (m) | sc-420484 | 20 µg | $397.00 |
Overview
Gap43 encodes growth associated protein 43 (GAP-43), a neuron-enriched, membrane-associated phosphoprotein that localizes to growth cones and presynaptic terminals where it regulates neurite outgrowth, axon guidance, and synaptic remodeling. GAP-43 function is tightly linked to actin cytoskeleton dynamics and calcium-dependent signaling, including PKC-mediated phosphorylation that modulates membrane interactions and plasticity-associated processes. In mouse, Gap43 expression increases during development and after neural injury, making it a widely used molecular marker of axonal growth and regenerative responses. Dysregulated GAP-43–dependent circuitry remodeling has been associated with altered synaptic connectivity in models of neurodevelopmental and neurodegenerative disorders, supporting its relevance for mechanistic studies of neuronal network function.
GAP-43 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gap43 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gap43 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gap43 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GAP-43 protein expression.
This CRISPR knockout system enables efficient generation of Gap43-deficient cell models for investigation of GAP-43 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gap43 exon(s) critical for GAP-43 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gap43 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GAP-43 CRISPR/Cas9 KO Plasmid (m) and GAP-43 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gap43 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GAP-43 HDR Plasmid (m) and GAP-43 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gap43 homology arms to support homology-directed repair at defined Gap43 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.