galectin-8 CRISPR/Cas9 KO Plasmid (m): sc-424987
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- galectin-8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the galectin-8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
galectin-8 CRISPR/Cas9 KO Plasmid (m) | sc-424987 | 20 µg | $397.00 |
Overview
Mouse Lgals8 encodes galectin-8, a tandem-repeat β-galactoside–binding lectin that recognizes glycans on cell-surface and extracellular matrix proteins to regulate cell adhesion, migration, and integrin-dependent signaling. Galectin-8 functions as a damage sensor by binding exposed host glycans on ruptured endomembranes, promoting selective autophagy and coordinating innate immune responses to intracellular stress and infection. Through lectin-mediated crosslinking and signaling, it modulates inflammatory cytokine networks and cellular homeostasis, intersecting with pathways controlling endocytosis, cytoskeletal dynamics, and vesicular trafficking. Dysregulated galectin-8 activity has been associated with altered inflammatory states, fibrosis-related remodeling, and tumor microenvironment biology, making it relevant for mechanistic studies of immune regulation and tissue pathology in mouse models.
galectin-8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lgals8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lgals8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lgals8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish galectin-8 protein expression.
This CRISPR knockout system enables efficient generation of Lgals8-deficient cell models for investigation of galectin-8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lgals8 exon(s) critical for galectin-8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lgals8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by galectin-8 CRISPR/Cas9 KO Plasmid (m) and galectin-8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lgals8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by galectin-8 HDR Plasmid (m) and galectin-8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lgals8 homology arms to support homology-directed repair at defined Lgals8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.