FUCA2 Double Nickase Plasmid (h): sc-405221-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FUCA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FUCA2 Double Nickase Plasmid (h) and FUCA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FUCA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FUCA2 Antibody (B-11): sc-514038
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FUCA2 Double Nickase Plasmid (h) | sc-405221-NIC | 20 µg | $410.00 | |||
FUCA2 Double Nickase Plasmid (h2) | sc-405221-NIC-2 | 20 µg | $410.00 |
FUCA2 encodes lysosomal α-L-fucosidase 2, an exoglycosidase that removes terminal fucose residues from N- and O-linked glycoconjugates and contributes to glycan catabolism and remodeling. By regulating turnover of fucosylated glycoproteins and glycolipids, FUCA2 influences lysosome-dependent clearance processes and broader glycosylation-linked signaling events at the cell surface. Altered fucosidase activity and fucosylation patterns have been associated with changes in inflammation, immune recognition, and extracellular matrix interactions, making FUCA2 a useful node for studying glycome regulation. Dysregulated expression or activity of fucosidases, including FUCA2, has been reported in contexts such as cancer biology and metabolic and immune-related phenotypes, supporting mechanistic investigations in relevant model systems.
FUCA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FUCA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FUCA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FUCA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FUCA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.