FOXF1 CRISPR/Cas9 KO Plasmid (h): sc-403521
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXF1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FOXF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXF1 CRISPR/Cas9 KO Plasmid (h) | sc-403521 | 20 µg | $397.00 |
Overview
FOXF1 (forkhead box F1) encodes a forkhead transcription factor that controls mesenchymal cell identity and epithelial–mesenchymal signaling during development, with prominent roles in lung and pulmonary vascular morphogenesis. FOXF1 regulates gene programs linked to extracellular matrix organization, cell migration, and smooth muscle/pericyte differentiation, integrating with developmental pathways such as Hedgehog, WNT/β-catenin, and TGF-β signaling. Altered FOXF1 dosage or regulatory disruption has been associated with congenital pulmonary and vascular abnormalities and has been investigated in contexts of aberrant stromal remodeling and tumor–microenvironment interactions. As a nuclear DNA-binding protein, FOXF1 provides a tractable entry point for dissecting transcriptional networks that coordinate tissue patterning and vascular stability.
FOXF1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOXF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOXF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOXF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FOXF1 protein expression.
This CRISPR knockout system enables efficient generation of FOXF1-deficient cell models for investigation of FOXF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOXF1 exon(s) critical for FOXF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOXF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FOXF1 CRISPR/Cas9 KO Plasmid (h) and FOXF1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOXF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FOXF1 HDR Plasmid (h) and FOXF1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOXF1 homology arms to support homology-directed repair at defined FOXF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.