Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Flotillin-2 Double Nickase Plasmid (m): sc-420382-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Flotillin-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Flotillin-2 Double Nickase Plasmid (m) and Flotillin-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Flot2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Flotillin-2 Antibody (B-6): sc-28320
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Flotillin-2 Double Nickase Plasmid (m) | sc-420382-NIC | 20 µg | $410.00 |
Flot2 encodes flotillin-2, a lipid raft–associated scaffolding protein enriched at the plasma membrane and endosomal compartments where it helps organize membrane microdomains. Flotillin-2 participates in clathrin-independent endocytosis, membrane trafficking, and signal transduction by coordinating receptor internalization and downstream kinase pathways, including MAPK and PI3K/AKT signaling. It also contributes to actin cytoskeleton dynamics, cell adhesion, and migration through interactions with caveolae-like domains and cytoskeletal regulators. Altered flotillin-2 expression or localization has been associated with dysregulated proliferation and invasion phenotypes in cancer biology and with perturbations in neuronal and immune cell signaling, supporting its relevance in mechanistic disease models.
Flotillin-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Flot2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Flot2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Flot2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Flot2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.