Flg Anticuerpo (M2F12) recomendado para detectarFlg isoform α de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)

Flg Anticuerpo (M2F12): sc-57132

Flg Anticuerpo (M2F12) clasificado 4.5 de 5 por 11.
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Ficha técnica

    • Anti-Flg Anticuerpo M2F12 es un monoclonal de ratón IgG2a (kappa light chain) Flg Anticuerpo proporcionado como 200 µg/ml
    • raised against the ectodomain of Flg isoform α of human origin
    • Flg Anticuerpo (M2F12) es recomendado para detectar Flg isoform α de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)
    • Flg Anticuerpo (M2F12) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Flg (M2F12): sc-57132.
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Can this antibody detect FGFR1 isoform beta? I'm trying to blot for endogenous FGFR1 in PC3 cell line, and I'm not getting a signal so I wonder if PC3 cells are expressing only beta form and the antibody cannot detect or something.

Preguntado por: Juko
Thank you very much for your question. This antibody was raised against Flg isoform α (ectodomain) of human origin. Unfortunately, we have not tested this antibody on PC3 cells. Our labs suggest as positive controls e.g. PC-12, HeLa, SK-N-SH, Neuro-2A and U-87 MG whole cell lysates (we do provide all these in case you are interested). Please contact our Technical Service Department by phone, e-mail or live chat in case you feel the WB results could be improved by changing the protocol settings. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application and get a signal at the expected mw size in a positive sample material, we will send you a replacement antibody free of charge.
Respondida por: Technical Support Europe
Fecha de publicación: 2017-09-18

Can Flg (M2F12): sc-57132 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Flg (M2F12): sc-57132 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-09-15
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Rated 5 de 5 por de work well in western blot at 1:200,ov90 cell Although it looked like just so so.But it actually worked very well, so it is good
Fecha de publicación: 2017-09-20
Rated 5 de 5 por de Immunofluorescence I used it (Flg (M2F12)) for immunofluorescence at LX-2 cell (dilution 1:200), and appeared blight image.
Fecha de publicación: 2017-07-05
Rated 5 de 5 por de Great antibody The antibody is excellent in Western Blotting and IHC
Fecha de publicación: 2017-03-22
Rated 5 de 5 por de Strong antibody for IHC and WB Nice specific staining on paraffin sections, very clean and strong results. It's also a great choice for Western blot! Smart choice!
Fecha de publicación: 2017-02-01
Rated 5 de 5 por de Pulito e molto specifico Ho ricevuto 10 µg di anticorpo in prova e sono riuscito a vedere un segnale di FGFR1 finalmente pulito, chiaro ed inconfutabile. Mi ha risolto un problema che andava avanti da mesi.
Fecha de publicación: 2017-01-02
Rated 4 de 5 por de Positive band detected in WB in K Positive band detected in WB in K-562 whole cell lysate. -SCBT Publication Review
Fecha de publicación: 2015-05-10
Rated 4 de 5 por de Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Fecha de publicación: 2015-04-06
Rated 4 de 5 por de Good band detected in WB in HeLa whole cell lysate Good band detected in WB in HeLa whole cell lysate. -SCBT QC
Fecha de publicación: 2015-02-22
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