FBPase Double Nickase Plasmid (h): sc-404693-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FBPase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FBPase Double Nickase Plasmid (h) and FBPase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: liver FBPase Antibody (B-10): sc-166298
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBPase Double Nickase Plasmid (h) | sc-404693-NIC | 20 µg | $410.00 | |||
FBPase Double Nickase Plasmid (h2) | sc-404693-NIC-2 | 20 µg | $410.00 |
FBP1 encodes fructose-1,6-bisphosphatase (FBPase), a rate-limiting gluconeogenic enzyme that hydrolyzes fructose-1,6-bisphosphate to fructose-6-phosphate, counterbalancing phosphofructokinase activity in glycolysis. In human liver and kidney, FBPase helps maintain systemic glucose homeostasis during fasting and integrates hormonal and allosteric control within carbohydrate metabolism. Altered FBP1 expression or activity has been linked to inborn errors of gluconeogenesis and to metabolic rewiring observed in multiple cancers, where changes in gluconeogenic flux intersect with redox balance and biosynthetic demands. Because FBP1 sits at a key branch point of carbon flux, it is frequently studied in pathways connecting energy metabolism, nutrient sensing, and transcriptional regulation.
FBPase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.