FAM20C Double Nickase Plasmid (h): sc-406736-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM20C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FAM20C Double Nickase Plasmid (h) and FAM20C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FAM20C. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM20C Double Nickase Plasmid (h) | sc-406736-NIC | 20 µg | $410.00 | |||
FAM20C Double Nickase Plasmid (h2) | sc-406736-NIC-2 | 20 µg | $410.00 |
FAM20C encodes a secreted Golgi-associated protein kinase that phosphorylates Ser-x-Glu/pSer motifs on many extracellular matrix and secreted proteins, shaping their maturation, trafficking, and mineral-binding properties. Through regulation of phosphoprotein processing and extracellular matrix organization, FAM20C influences osteogenic differentiation, dentin formation, and broader biomineralization programs. Disruption of FAM20C activity is linked to heritable mineralization disorders and skeletal phenotypes, and altered kinase activity has been examined in contexts of tissue remodeling and tumor-associated extracellular matrix changes. As a central component of the secretory pathway phosphoproteome, FAM20C is relevant for studies of Golgi kinase signaling, secreted phosphoproteomics, and matrix-driven cell behavior.
FAM20C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FAM20C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FAM20C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FAM20C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FAM20C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.