Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ets-2 Double Nickase Plasmid (h): sc-416381-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ets-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ets-2 Double Nickase Plasmid (h) and Ets-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ETS2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ets-2 Antibody (E-5): sc-365666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ets-2 Double Nickase Plasmid (h) | sc-416381-NIC | 20 µg | $410.00 | |||
Ets-2 Double Nickase Plasmid (h2) | sc-416381-NIC-2 | 20 µg | $410.00 |
ETS2 encodes Ets-2, a member of the ETS family of sequence-specific transcription factors that binds GGAA/T motifs to regulate gene expression programs controlling cell-cycle progression, differentiation, and survival. Ets-2 activity is modulated by MAPK/ERK-dependent phosphorylation and integrates signals downstream of growth factor and cytokine pathways to shape transcriptional responses in diverse cell types. Through regulation of genes involved in extracellular matrix remodeling, inflammatory signaling, and proliferation, ETS2 has been implicated in context-dependent oncogenic and tumor-suppressive networks, as well as immune and developmental phenotypes. Altered ETS2 dosage or pathway activation has been associated with malignancies and chromosome 21–linked biology, making it a useful node for mechanistic studies of transcriptional regulation and signaling crosstalk.
Ets-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ETS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ETS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ETS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ETS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.