ERK 1/2 Anticuerpo (MK1) recomendado para detectarERK 1 and ERK 2 de mouse, rat, human y canine origen, mediante WB, IP y IF

ERK 1/2 Anticuerpo (MK1): sc-135900

ERK 1/2 Anticuerpo (MK1) clasificado 4.4 de 5 por 14.
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Ficha técnica

    • ERK 1/2 Anticuerpo (MK1) es un monoclonal de ratón mouse IgG1 κ (kappa light chain) proporcionado como 50 µg/0.5 ml
    • producido contra los amino ácidos 325-345 de ERK 1 de origen rat
    • recomendado para detectarERK 1 and ERK 2 de mouse, rat, human y canine origen, mediante WB, IP y IF
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Ver ERK 2 (D-2): sc-1647 para ERK 1/2 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de ERK 1/2 (MK1): sc-135900.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Preguntado por: Anónimo
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Support
Fecha de publicación: 2016-12-22

Will this ERK1/2 antibody detect the protein in bovine samples?

Preguntado por: Anónimo
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Respondida por: Technical Support
Fecha de publicación: 2016-12-22

Can ERK2 (SC-135900) be used as a loading control for western blot lysates?

Preguntado por: Eldhose
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Rated 5 de 5 por de Excellent antibody for WBs I have used this antibody at 1:1000 dilution for western blotting using a number of human cell lines and obtained a very strong signal. Extremely pleased with this antibody.
Fecha de publicación: 2019-06-15
Rated 1 de 5 por de No signal in In-Cell Western This antibody did not work for my In-Cell Western assays in HEK293. I use 4% paraformaldehyde fixation and methanol permeabilization, and I don't know if it could work with a different protocol. I would not purchase this antibody for any immunocytochemistry approaches.
Fecha de publicación: 2018-05-07
Rated 5 de 5 por de good result This antibody can be used to publish using the antibody in WB and IHC in mouse neuron.
Fecha de publicación: 2017-05-28
Rated 5 de 5 por de good ERK 1/2 antibody worked well in the rat cerebrum tissue for WB analysis
Fecha de publicación: 2017-05-13
Rated 5 de 5 por de Works very well It was used for WB on rat cerebrum tissue extract., which shows good quality and got bands very clearly.
Fecha de publicación: 2017-04-24
Rated 5 de 5 por de positive band Dilution detection 1:1000. It shows the positive band, I am satisfied with the result.
Fecha de publicación: 2017-02-26
Rated 3 de 5 por de Low sensitivity for WB This ab has good specificity, but relatively low sensitivity for WB with ERK 1/2 from human, mouse and rat.
Fecha de publicación: 2017-01-30
Rated 4 de 5 por de band is clear Western work well in human protein samples.Do not detect the antibody in IHC of IF
Fecha de publicación: 2016-12-29
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