EphB6 CRISPR/Cas9 KO Plasmid (m): sc-420202
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EphB6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EphB6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EphB6 Antibody (D-7): sc-398795
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EphB6 CRISPR/Cas9 KO Plasmid (m) | sc-420202 | 20 µg | $397.00 |
Overview
Ephb6 encodes EphB6, a member of the Eph receptor tyrosine kinase family that participates in contact-dependent signaling with ephrin-B ligands to regulate cell–cell communication. Although EphB6 has impaired kinase activity relative to other EphB receptors, it modulates receptor clustering and downstream pathways controlling cytoskeletal remodeling, cell adhesion, and directional migration, with links to Rho family GTPase signaling and MAPK/PI3K network crosstalk. In mouse systems, EphB6 is studied in processes such as immune cell activation and tissue organization where ephrin–Eph gradients shape cell positioning. Dysregulated EphB6-associated signaling has been investigated in contexts of altered epithelial behavior and tumor-related phenotypes, supporting its use as a mechanistic node in studies of growth control and microenvironmental interactions.
EphB6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ephb6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ephb6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ephb6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EphB6 protein expression.
This CRISPR knockout system enables efficient generation of Ephb6-deficient cell models for investigation of EphB6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ephb6 exon(s) critical for EphB6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ephb6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EphB6 CRISPR/Cas9 KO Plasmid (m) and EphB6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ephb6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EphB6 HDR Plasmid (m) and EphB6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ephb6 homology arms to support homology-directed repair at defined Ephb6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.