Emi1 Antibody (B-3): sc-365212

Emi1 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Emi1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Emi1 antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Emi1 protein, known as early mitotic inhibitor 1, plays a crucial role in regulating mitosis by inhibiting the anaphase-promoting complex/cyclosome (APC), which is essential for timely progression of the cell cycle. This inhibition prevents premature degradation of cyclins, allowing for proper cell cycle transitions, particularly cyclin A accumulation and entry into S phase. Emi1 is transcriptionally induced at G1-S transition by E2F transcription factor, and overexpression can accelerate S phase entry, even overriding G1 phase blocks caused by Cdh1 or retinoblastoma protein (pRb). Furthermore, Emi1 depletion through RNA interference inhibits S phase entry by preventing cyclin A accumulation, highlighting Emi1′s importance in cell cycle regulation. Emi1 maintains metaphase II arrest in unfertilized eggs, suggesting a potential role as a mediator of cytostatic factor activity. Notably, human Emi1 shares significant similarity with Xenopus laevis Emi1, which also inhibits APC (Cdc20) ubiquitination complex, underscoring this regulatory mechanism′s conserved nature across species.

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Emi1 Antibody (B-3) References:

1. Identification of a family of human F-box proteins.  |  Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
2. Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors.  |  Chiaur, DS., et al. 2000. Cytogenet Cell Genet. 88: 255-8. PMID: 10828603
3. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.  |  Reimann, JD., et al. 2001. Cell. 105: 645-55. PMID: 11389834
4. Emi1 is required for cytostatic factor arrest in vertebrate eggs.  |  Reimann, JD. and Jackson, PK. 2002. Nature. 416: 850-4. PMID: 11976684
5. Systematic analysis and nomenclature of mammalian F-box proteins.  |  Jin, J., et al. 2004. Genes Dev. 18: 2573-80. PMID: 15520277
6. The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1.  |  Eldridge, AG., et al. 2006. Cell. 124: 367-80. PMID: 16439210
7. Mouse emi1 has an essential function in mitotic progression during early embryogenesis.  |  Lee, H., et al. 2006. Mol Cell Biol. 26: 5373-81. PMID: 16809773
8. Overexpression of the anaphase promoting complex/cyclosome inhibitor Emi1 leads to tetraploidy and genomic instability of p53-deficient cells.  |  Lehman, NL., et al. 2006. Cell Cycle. 5: 1569-73. PMID: 16861914
9. Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor.  |  Miller, JJ., et al. 2006. Genes Dev. 20: 2410-20. PMID: 16921029
10. Defining the role of Emi1 in the DNA replication-segregation cycle.  |  Di Fiore, B. and Pines, J. 2008. Chromosoma. 117: 333-8. PMID: 18317792
11. Examining the mechanistic relationship of APC/CCDH1 and its interphase inhibitor EMI1.  |  Bolhuis, DL., et al. 2022. Protein Sci. 31: e4324. PMID: 35634770
12. Expression significance of Emi1, UBCH10 and CyclinB1 in esophageal squamous cell carcinoma.  |  Li, H., et al. 2023. Pathol Oncol Res. 29: 1611081. PMID: 37168048

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Emi1 Antibody (B-3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Emi1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Emi1 Antibody (B-3) Specifications

Alternate Product Name: Emi1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 148-447

Epitope Target: Emi1

Epitope Species: human

Product Reactivity/Detection: Emi1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Emi1 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin