ELOVL4 Double Nickase Plasmid (h): sc-407562-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ELOVL4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ELOVL4 Double Nickase Plasmid (h) and ELOVL4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ELOVL4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ELOVL4 Double Nickase Plasmid (h) | sc-407562-NIC | 20 µg | $410.00 | |||
ELOVL4 Double Nickase Plasmid (h2) | sc-407562-NIC-2 | 20 µg | $410.00 |
ELOVL4 encodes an endoplasmic reticulum–localized elongase that catalyzes the synthesis of very long-chain fatty acids, including substrates required for specialized lipids such as retinal and epidermal lipids. By extending long-chain acyl-CoAs, ELOVL4 contributes to lipid homeostasis, membrane composition, and barrier function, influencing pathways linked to sphingolipid and phospholipid metabolism. Perturbation of ELOVL4 activity alters very long-chain lipid pools and has been associated with inherited retinal degeneration phenotypes and neurocutaneous disorders, highlighting its relevance to photoreceptor integrity and skin physiology. As a metabolic node at the interface of fatty acid elongation and organelle lipid remodeling, ELOVL4 is commonly studied in contexts of lipid stress, membrane biophysics, and tissue-specific lipid requirements.
ELOVL4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ELOVL4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ELOVL4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ELOVL4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ELOVL4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.