Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Elongin B CRISPR/Cas9 KO Plasmid (h): sc-402154
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Elongin B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Elongin B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Elongin B Antibody (D-5): sc-133090
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Elongin B CRISPR/Cas9 KO Plasmid (h) | sc-402154 | 20 µg | $397.00 |
Overview
ELOB encodes Elongin B, a core component of the Elongin BC complex that functions as an adaptor in E3 ubiquitin ligase assemblies and supports transcriptional regulation through interactions with elongation factors. In the von Hippel–Lindau (VHL) ubiquitin ligase complex, Elongin B helps scaffold substrate recognition and ubiquitination processes that influence hypoxia signaling, proteostasis, and cellular stress responses. Through these roles, ELOB contributes to regulation of protein turnover and transcription-coupled pathways implicated in tumor biology and cellular adaptation to microenvironmental cues. Perturbation of Elongin BC–associated complexes is frequently examined in studies of oncogenic signaling, ubiquitin-dependent degradation networks, and transcriptional control.
Elongin B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELOB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELOB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELOB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Elongin B protein expression.
This CRISPR knockout system enables efficient generation of ELOB-deficient cell models for investigation of Elongin B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELOB exon(s) critical for Elongin B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELOB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Elongin B CRISPR/Cas9 KO Plasmid (h) and Elongin B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELOB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Elongin B HDR Plasmid (h) and Elongin B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELOB homology arms to support homology-directed repair at defined ELOB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.