eIF4E3 CRISPR/Cas9 KO Plasmid (h): sc-407113
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4E3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF4E3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4E3 CRISPR/Cas9 KO Plasmid (h) | sc-407113 | 20 µg | $397.00 |
Overview
EIF4E3 encodes eIF4E3, a member of the eukaryotic translation initiation factor 4E family that recognizes the 5′ mRNA cap and contributes to regulation of cap-dependent translation initiation. Compared with canonical eIF4E1, eIF4E3 is often described as a noncanonical cap-binding protein that can influence mRNA-selective translation programs, shaping proteostasis and stress-adaptive gene expression. Through its impact on translational control, eIF4E3 intersects with growth and nutrient-sensing pathways such as mTOR signaling and broader RNA metabolism networks. Dysregulation of cap-dependent translation is a recurring feature of oncogenic and stress-response states, making EIF4E3 a useful target for mechanistic studies of translational rewiring in disease-relevant cellular models.
eIF4E3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EIF4E3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EIF4E3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EIF4E3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF4E3 protein expression.
This CRISPR knockout system enables efficient generation of EIF4E3-deficient cell models for investigation of eIF4E3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EIF4E3 exon(s) critical for eIF4E3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EIF4E3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF4E3 CRISPR/Cas9 KO Plasmid (h) and eIF4E3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EIF4E3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF4E3 HDR Plasmid (h) and eIF4E3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EIF4E3 homology arms to support homology-directed repair at defined EIF4E3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.