EIF2D Double Nickase Plasmid (h): sc-405379-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EIF2D Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EIF2D Double Nickase Plasmid (h) and EIF2D Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF2D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF2D Antibody (2D10): sc-293396
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EIF2D Double Nickase Plasmid (h) | sc-405379-NIC | 20 µg | $410.00 | |||
EIF2D Double Nickase Plasmid (h2) | sc-405379-NIC-2 | 20 µg | $410.00 |
EIF2D encodes eukaryotic translation initiation factor 2D, a noncanonical initiation factor implicated in alternative translation initiation and tRNA delivery to the ribosomal P site under specific cellular contexts. Through its role in translational control, EIF2D intersects with pathways governing proteostasis and adaptive responses to cellular stress, including modules linked to eIF2 signaling and integrated stress response dynamics. Perturbation of translation initiation and stress-adaptive protein synthesis is broadly relevant to mechanisms studied in neurodegeneration, cancer cell plasticity, and viral infection models, where altered translational programs can reshape cell-state transitions. As a result, EIF2D is frequently investigated for how shifts in initiation factor usage influence mRNA-specific translation and downstream phenotypes.
EIF2D Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF2D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF2D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF2D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF2D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.