EHHADH Double Nickase Plasmid (h): sc-404068-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EHHADH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EHHADH Double Nickase Plasmid (h) and EHHADH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EHHADH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EHHADH Antibody (D-2): sc-393123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EHHADH Double Nickase Plasmid (h) | sc-404068-NIC | 20 µg | $410.00 | |||
EHHADH Double Nickase Plasmid (h2) | sc-404068-NIC-2 | 20 µg | $410.00 |
EHHADH encodes enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase, a bifunctional peroxisomal enzyme that catalyzes sequential steps of fatty acid β-oxidation, particularly for very-long-chain and branched-chain substrates. By supporting peroxisomal lipid catabolism and redox balance, EHHADH influences cellular energy homeostasis and lipid signaling pathways that intersect with mitochondrial metabolism. Altered EHHADH expression or activity has been associated with metabolic dysregulation in liver and kidney, and is frequently studied in contexts such as peroxisome-related disorders, steatosis, and renal injury models.
EHHADH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EHHADH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EHHADH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EHHADH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EHHADH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.