eHAND CRISPR Activation Plasmid (h): sc-403311-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eHAND CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- eHAND CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by eHAND CRISPR Activation Plasmid (h) and eHAND CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the HAND1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eHAND Antibody (F-7): sc-390376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eHAND CRISPR Activation Plasmid (h) | sc-403311-ACT | 20 µg | $397.00 |
HAND1 encodes the basic helix–loop–helix transcription factor eHAND, a key regulator of mesoderm patterning and cardiovascular development. eHAND coordinates gene expression programs that control lineage commitment, cell migration, and morphogen-driven transcriptional networks, including interactions with E-box–containing regulatory elements and crosstalk with developmental signaling pathways. In human biology, altered HAND1 activity has been linked to congenital heart development defects and placental/trophoblast abnormalities, making it a useful node for studying cardiogenesis and early embryonic gene regulatory circuitry.
eHAND CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous HAND1 expression without altering the underlying DNA sequence.
eHAND CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the HAND1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the HAND1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous eHAND expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native HAND1 locus and enabling the study of eHAND-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of eHAND pathway restoration in tumor cells with silenced or reduced HAND1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.