Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EDG-1 Antibody (A-6): sc-48356. Western blot analysis of EDG-1 expression in human hippocampus (A), human cerebral cortex (B), human hypothalamus (C) and human cerebellum (D) tissue extracts.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h): sc-400413
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EDG-1/S1P1/S1PR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EDG-1/S1P1/S1PR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EDG-1/S1P1/S1PR1 Antibody (A-6): sc-48356
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h) | sc-400413 | 20 µg | $397.00 |