Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dyrk1A Double Nickase Plasmid (h): sc-401928-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dyrk1A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dyrk1A Double Nickase Plasmid (h) and Dyrk1A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DYRK1A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dyrk1A Antibody (RR.7): sc-100376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dyrk1A Double Nickase Plasmid (h) | sc-401928-NIC | 20 µg | $410.00 | |||
Dyrk1A Double Nickase Plasmid (h2) | sc-401928-NIC-2 | 20 µg | $410.00 |
DYRK1A encodes dual-specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1A), a serine/threonine kinase that undergoes autophosphorylation and modulates phosphorylation networks controlling cell-cycle progression, neuronal differentiation, and protein stability. Dyrk1A influences transcriptional programs and signaling cascades through phosphorylation of substrates involved in chromatin regulation, RNA processing, and proteostasis, linking it to developmental and stress-response pathways. In human biology, altered DYRK1A dosage and activity have been associated with neurodevelopmental phenotypes and dosage-sensitive gene networks, and dysregulation of its signaling outputs has been examined in the context of proliferative and synaptic processes. These features make DYRK1A a frequently studied node for dissecting kinase-dependent control of gene expression and cell fate decisions.
Dyrk1A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DYRK1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DYRK1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DYRK1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DYRK1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.