Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dynein IC1, cytosolic Double Nickase Plasmid (h): sc-405213-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynein IC1, cytosolic Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dynein IC1, cytosolic Double Nickase Plasmid (h) and Dynein IC1, cytosolic Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DYNC1I1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynein IC1, cytosolic Antibody (G-1): sc-515227
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynein IC1, cytosolic Double Nickase Plasmid (h) | sc-405213-NIC | 20 µg | $410.00 | |||
Dynein IC1, cytosolic Double Nickase Plasmid (h2) | sc-405213-NIC-2 | 20 µg | $410.00 |
DYNC1I1 encodes the cytosolic dynein 1 intermediate chain 1, a core subunit of the dynein–dynactin motor complex that powers minus-end–directed transport along microtubules. Dynein IC1 contributes to cargo recognition and motor regulation, supporting endosome and lysosome positioning, Golgi organization, mitotic spindle dynamics, and retrograde axonal transport in neurons. Through these processes, DYNC1I1 helps coordinate intracellular trafficking, cell division, and spatial signaling from organelles. Dysregulation of dynein complex components is frequently studied in the context of neurodevelopmental and neurodegenerative mechanisms, as well as chromosomal instability and altered proteostasis pathways.
Dynein IC1, cytosolic Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DYNC1I1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DYNC1I1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DYNC1I1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DYNC1I1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.