Doublecortin Double Nickase Plasmid (h): sc-400033-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Doublecortin/DCX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Doublecortin/DCX Double Nickase Plasmid (h) and Doublecortin/DCX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DCX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Doublecortin/DCX Antibody (E-6): sc-271390
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Doublecortin Double Nickase Plasmid (h) | sc-400033-NIC | 20 µg | $410.00 | |||
Doublecortin Double Nickase Plasmid (h2) | sc-400033-NIC-2 | 20 µg | $410.00 |
DCX encodes Doublecortin, a microtubule-associated protein that binds and stabilizes microtubules to regulate neuronal migration, neurite outgrowth, and cortical layer formation during brain development. Doublecortin coordinates cytoskeletal remodeling and centrosome-to-nucleus coupling, integrating with microtubule dynamics that shape cell polarity and directed movement. Disruption of DCX perturbs radial migration and neuronal positioning, and pathogenic variants are linked to neurodevelopmental malformations including X-linked lissencephaly and subcortical band heterotopia. As a DCX marker and functional regulator in immature neurons, it is widely used to study mechanisms of neurogenesis, cytoskeletal regulation, and developmental circuit assembly.
Doublecortin/DCX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DCX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DCX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DCX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DCX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.