Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dnmt3a Double Nickase Plasmid (m): sc-420035-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dnmt3a Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dnmt3a Double Nickase Plasmid (m) and Dnmt3a Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dnmt3a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dnmt3a Antibody (C-12): sc-365769
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dnmt3a Double Nickase Plasmid (m) | sc-420035-NIC | 20 µg | $410.00 | |||
Dnmt3a Double Nickase Plasmid (m2) | sc-420035-NIC-2 | 20 µg | $410.00 |
Mouse Dnmt3a encodes a de novo DNA methyltransferase that establishes CpG methylation patterns during early development and lineage specification, shaping stable gene expression programs and chromatin states. DNMT3A functions within epigenetic silencing networks that interface with histone modifications and transcription factor occupancy to regulate differentiation, imprinting, and transposon repression. Disruption of Dnmt3a perturbs methylome integrity and cell fate decisions, making it a key node for studying epigenetic memory, stem cell biology, and hematopoietic regulation. Altered DNMT3A activity is widely used as a mechanistic entry point for modeling methylation-associated dysregulation observed in cancer and developmental disorders.
Dnmt3a Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dnmt3a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dnmt3a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dnmt3a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dnmt3a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.