Dicer Double Nickase Plasmid (h): sc-400365-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dicer Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dicer Double Nickase Plasmid (h) and Dicer Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DICER1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dicer Antibody (F-10): sc-136979
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dicer Double Nickase Plasmid (h) | sc-400365-NIC | 20 µg | $410.00 | |||
Dicer Double Nickase Plasmid (h2) | sc-400365-NIC-2 | 20 µg | $410.00 |
DICER1 encodes Dicer, an RNase III endonuclease essential for microRNA and siRNA biogenesis through processing of precursor hairpins into ~21–23 nt small RNAs. By loading these small RNAs into Argonaute-containing RISC complexes, Dicer regulates post-transcriptional gene silencing and impacts pathways controlling differentiation, proliferation, and stress responses. DICER1-dependent small RNA networks influence genome stability and innate immune signaling through modulation of repetitive element activity and double-strand break responses. Dysregulation or mutation of DICER1 perturbs microRNA homeostasis and is linked to altered gene-expression programs in multiple disease contexts, making it a key target for mechanistic studies of RNA interference and transcriptome control.
Dicer Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DICER1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DICER1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DICER1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DICER1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.