Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DGUOK CRISPR/Cas9 KO Plasmid (h): sc-407916
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DGUOK CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DGUOK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DGUOK Antibody (H-3): sc-376267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGUOK CRISPR/Cas9 KO Plasmid (h) | sc-407916 | 20 µg | $397.00 |
Overview
Deoxyguanosine kinase (DGUOK) is a mitochondrial deoxyribonucleoside kinase that catalyzes phosphorylation of purine deoxyribonucleosides, supporting the mitochondrial deoxynucleotide pool required for mtDNA replication and repair. It functions within mitochondrial nucleotide salvage pathways and contributes to genome maintenance, oxidative phosphorylation capacity, and cellular bioenergetic homeostasis. Altered DGUOK activity is linked to mitochondrial DNA depletion phenotypes and perturbed mitochondrial function, making it relevant for studies of mitochondrial disorders, hepatocerebral syndromes, and energy metabolism defects in human cells.
DGUOK CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DGUOK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DGUOK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DGUOK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DGUOK protein expression.
This CRISPR knockout system enables efficient generation of DGUOK-deficient cell models for investigation of DGUOK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DGUOK exon(s) critical for DGUOK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DGUOK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DGUOK CRISPR/Cas9 KO Plasmid (h) and DGUOK CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DGUOK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DGUOK HDR Plasmid (h) and DGUOK HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DGUOK homology arms to support homology-directed repair at defined DGUOK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.