DGK-alpha Anticuerpo (C-11) recomendado para detectarDGK-α de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

DGK-α Anticuerpo (C-11): sc-271644

DGK-α Anticuerpo (C-11) clasificado 4.8 de 5 por 4.
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Ficha técnica

    • Anti-DGK-alpha Anticuerpo C-11 es un monoclonal de ratón IgG1 κ DGK-α Anticuerpo, ver las 3 publicaciones, proporcionado como 200 µg/ml
    • raised against amino acids 242-298 mapping within an internal region of DGK-α of human origin
    • DGK-alpha Anticuerpo (C-11) es recomendado para detectar DGK-α de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • DGK-alpha Anticuerpo (C-11) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de DGK-α (C-11): sc-271644.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DGK-α Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with DGK-α Antibody (C-11) (see ordering information below).
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I have ordered DGK-α Antibody (C-11) Alexa Fluor® 488, sc-271644 AF488 from you and want to try a flow cytometry with this antibody. I wonder do you have the concentration for this antibody and which staining I should use: intracellular or surface?

Preguntado por: Janny
Thank you for your question. sc-271644 AF488 is provided at a concentration of 200 µg in 1 mL. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. Based on IF and IHC(P) staining using the antibody, we observed both cytoplasmic and nuclear staining. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Respondida por: Technical Support
Fecha de publicación: 2020-02-26

I am using DGK-α (C-11): sc-271644 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-03-01
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Rated 4 de 5 por de Great antibody for WB Fine signal and clean background from this antibody!
Fecha de publicación: 2017-05-16
Rated 5 de 5 por de Highly recommended Highly recommended mouse monoclonal. Strong and specific signal produced by Western blot, IHC and IF
Fecha de publicación: 2017-01-27
Rated 5 de 5 por de Robust band observed in WB in Jurkat whole Robust band observed in WB in Jurkat whole cell lysate. -SCBT QC
Fecha de publicación: 2015-06-18
Rated 5 de 5 por de Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Fecha de publicación: 2015-02-26
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